Introduction:
Ethidium Bromide is a dark red, crystalline powder widely known as a fluorescent intercalating agent used for nucleic acid staining. It binds specifically to DNA and RNA, allowing visualization of genetic material under ultraviolet (UV) light. Ethidium Bromide is highly effective and sensitive, making it a preferred dye for gel electrophoresis and molecular biology research applications.
 
Applications:
Molecular Biology Laboratories: Used as a fluorescent stain for DNA and RNA in agarose and polyacrylamide gels.
Genetic Research: Helps in visualization and quantification of nucleic acids.
Biotechnology: Applied in various DNA extraction and electrophoretic separation processes.
Forensic Science: Utilized in DNA fingerprinting and analytical studies.
Educational Institutions: Used in molecular biology teaching experiments involving gel electrophoresis.
 
Benefits:
Provides clear and sharp visualization of nucleic acids under UV light.
Highly sensitive for detecting small amounts of DNA or RNA.
Cost-effective and easy to use in standard laboratory procedures.
Stable in powder form with long shelf life.
Compatible with a wide range of electrophoresis buffers and gel systems.