Introduction:
Ethidium Bromide is a dark red crystalline powder widely recognized as a fluorescent dye and intercalating agent used for detecting and visualizing nucleic acids. It binds strongly to DNA and RNA molecules and fluoresces under ultraviolet (UV) light, making it an essential reagent in molecular biology, genetic engineering, and biochemical research. Known for its high sensitivity and reliability, Ethidium Bromide is a standard tool for DNA electrophoresis and related laboratory techniques.
 
Applications:
Molecular Biology: Used as a fluorescent stain for DNA and RNA in agarose or polyacrylamide gels.
Genetic Research: Helps in visualization and quantification of nucleic acids in gel electrophoresis.
Forensic Science: Used in DNA fingerprinting and identification applications.
Biotechnology: Applied in plasmid DNA isolation, cloning, and sequencing workflows.
Educational & Research Laboratories: Commonly used for training and analytical studies involving nucleic acid detection.
 
Benefits:
Highly sensitive for detecting even trace amounts of DNA/RNA.
Produces bright, distinct fluorescence under UV light.
Reliable and cost-effective for routine lab use.
Long shelf life and stable under normal storage conditions.
Works efficiently across a wide range of gel systems and buffers.